› 8yib

X-ray diffraction
2.27Å resolution

Staphylococcus aureus lipase -PSA complex - covalent bonding state

Released:
DOI: 10.2210/pdb8yib/pdb
PDB entry 8yib coloured by chain, front view.
PDB entry 8yib coloured by chain, side view.
PDB entry 8yib coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structure of Staphylococcus aureus lipase complex with unsaturated petroselinic acid.
Kitadokoro J, Kamitani S, Okuno Y, Hikima T, Yamamoto M, Hirokawa T, Kitadokoro K
OpenAccess logo FEBS Open Bio (2024)
PMID: 38757397

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 408 amino acids
Theoretical weight: 45.8 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0U1MWF9 (Residues: 295-690; Coverage: 57%)
Gene names: BN1321_80040, lip

Ligands and Environments

13 bound ligands:
Ligand FMT 10 x FMT
Ligand OCA 4 x OCA
Ligand PPI 1 x PPI
Ligand ACY 1 x ACY
Ligand 4I1 2 x 4I1
Ligand BUA 2 x BUA
Ligand DAO 4 x DAO
Ligand ZN 2 x ZN
Ligand CA 2 x CA
Ligand MG 1 x MG
Ligand GOL 5 x GOL
Ligand 6NA 3 x 6NA
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P4122
Unit cell:
a: 132.548Å b: 132.548Å c: 249.754Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.221
Expression system: Escherichia coli

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