› 8yky

Electron Microscopy
2.99Å resolution

Structure of human class T GPCR TAS2R14-Ggustducin complex with agonist 28.1

Released:
DOI: 10.2210/pdb8yky/pdb
PDB entry 8yky coloured by chain, front view.
PDB entry 8yky coloured by chain, side view.
PDB entry 8yky coloured by chain, top view.
Source organisms:
Primary publication:
Bitter taste TAS2R14 activation by intracellular tastants and cholesterol.
Hu X, Ao W, Gao M, Wu L, Pei Y, Liu S, Wu Y, Zhao F, Sun Q, Liu J, Jiang L, Wang X, Li Y, Tan Q, Cheng J, Yang F, Yang C, Sun J, Hua T, Liu ZJ
Nature (2024)
PMID: 38776963
Related structures: EMD-39376

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-246947 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
G alpha gustducin protein Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 42.37 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 366 amino acids
Theoretical weight: 39.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
ScFv16 protein Chain: S
Molecule details ›
Chain: S
Length: 286 amino acids
Theoretical weight: 30.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Taste receptor type 2 member 14; exo-alpha-sialidase Chain: R
Molecule details ›
Chain: R
Length: 990 amino acids
Theoretical weight: 110.84 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q59310 (Residues: 243-694; Coverage: 65%)
  • Canonical: Q9NYV8 (Residues: 2-317; Coverage: 100%)
Gene names: TAS2R14, nanH
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand A1AEI 1 x A1AEI
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.99Å
Relevant EMDB volumes: EMD-39376
Expression system: Spodoptera frugiperda

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