› 8yl8

X-ray diffraction
2.21Å resolution

Crystal structure of the de novo designed protein 200 AA in the crystal form 2

Released:
DOI: 10.2210/pdb8yl8/pdb
PDB entry 8yl8 coloured by chain, front view.
PDB entry 8yl8 coloured by chain, side view.
PDB entry 8yl8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Scalable protein design using optimization in a relaxed sequence space.
Frank C, Khoshouei A, Fuβ L, Schiwietz D, Putz D, Weber L, Zhao Z, Hattori M, Feng S, de Stigter Y, Ovchinnikov S, Dietz H
Science 386 439-445 (2024)
PMID: 39446959

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-278613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
De novo protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 211 amino acids
Theoretical weight: 23.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: P212121
Unit cell:
a: 88.007Å b: 90.27Å c: 130.015Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.244
Expression system: Escherichia coli

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