› 8ynm

Electron Microscopy
3.49Å resolution

Structure of the Caspase-8/cFLIP death effector domain assembly

Released:
DOI: 10.2210/pdb8ynm/pdb
PDB entry 8ynm coloured by chain, front view.
PDB entry 8ynm coloured by chain, side view.
PDB entry 8ynm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Reverse hierarchical DED assembly in the cFLIP-procaspase-8 and cFLIP-procaspase-8-FADD complexes.
Yang CY, Tseng YC, Tu YF, Kuo BJ, Hsu LC, Lien CI, Lin YS, Wang YT, Lu YC, Su TW, Lo YC, Lin SC
Nat Commun 15 8974 (2024)
PMID: 39419969
Related structures: EMD-39427

Function and Biology Details

Reaction catalysed: 
Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-280509 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CASP8 and FADD-like apoptosis regulator subunit p43 Chains: F, G, H, I, J, K, N, O
Molecule details ›
Chains: F, G, H, I, J, K, N, O
Length: 181 amino acids
Theoretical weight: 20.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15519 (Residues: 1-181; Coverage: 38%)
Gene names: CASH, CASP8AP1, CFLAR, CLARP, MRIT
Sequence domains: Death effector domain
Caspase-8 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 479 amino acids
Theoretical weight: 55.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14790 (Residues: 1-479; Coverage: 100%)
Gene names: CASP8, MCH5
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.49Å
Relevant EMDB volumes: EMD-39427
Expression system: Escherichia coli

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