› 8yon

Electron Microscopy
6.73Å resolution

structure of phage T6 full-length topoisomerase II bound with DNA

Released:
DOI: 10.2210/pdb8yon/pdb
PDB entry 8yon coloured by chain, front view.
PDB entry 8yon coloured by chain, side view.
PDB entry 8yon coloured by chain, top view.
Source organisms:
Entry authors: Chen YT, Xin YH, Xian RQ
Related structures: EMD-39454

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269222 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA topoisomerase medium subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 452 amino acids
Theoretical weight: 51.95 KDa
Source organism: Escherichia phage T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P07065 (Residues: 1-442; Coverage: 100%)
Gene name: 52
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
DNA topoisomerase (ATP-hydrolyzing) Chains: C, D
Molecule details ›
Chains: C, D
Length: 611 amino acids
Theoretical weight: 69.24 KDa
Source organism: Enterobacteria phage T6
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A346FJ89 (Residues: 1-605; Coverage: 100%)
Gene name: EcT6_00003
Sequence domains:
DNA (50-MER) Chain: E
Molecule details ›
Chain: E
Length: 52 nucleotides
Theoretical weight: 16.06 KDa
DNA (50-MER) Chain: F
Molecule details ›
Chain: F
Length: 52 nucleotides
Theoretical weight: 15.97 KDa

Ligands and Environments

1 bound ligand:
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.73Å
Relevant EMDB volumes: EMD-39454
Expression system: Escherichia coli

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