› 8z76

X-ray diffraction
1.8Å resolution

The structure of thiocyanate dehydrogenase from Pelomicrobium methylotrophicum (pmTcDH), activated by crystals soaking with 1 mM CuCl2 during 6 months

Released:
DOI: 10.2210/pdb8z76/pdb
PDB entry 8z76 coloured by chain, front view.
PDB entry 8z76 coloured by chain, side view.
PDB entry 8z76 coloured by chain, top view.
Source organism: Pelomicrobium methylotrophicum
Entry authors: Varfolomeeva LA, Solovieva AY, Shipkov NS, Dergousova NI, Boyko KM, Tikhonova TV, Popov VO

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-228417 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Twin-arginine translocation signal domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 489 amino acids
Theoretical weight: 54.09 KDa
Source organism: Pelomicrobium methylotrophicum
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A5C7ETD9 (Residues: 46-513; Coverage: 91%)
Gene name: FR698_09980

Ligands and Environments

3 bound ligands:
Ligand EDO 5 x EDO
Ligand CU 10 x CU
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17UM
Spacegroup: C2221
Unit cell:
a: 97.8Å b: 101.75Å c: 276.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.217
Expression system: Escherichia coli

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