› 8z8j

Electron Microscopy
3.16Å resolution

Cryo-EM structure of Thogoto virus polymerase in transcription pre-initiation conformation 2

Released:
DOI: 10.2210/pdb8z8j/pdb
PDB entry 8z8j coloured by chain, front view.
PDB entry 8z8j coloured by chain, side view.
PDB entry 8z8j coloured by chain, top view.
Source organism: Thogoto virus (isolate SiAr 126)
Primary publication:
Cryo-EM structures of Thogoto virus polymerase reveal unique RNA transcription and replication mechanisms among orthomyxoviruses.
Xue L, Chang T, Li Z, Wang C, Zhao H, Li M, Tang P, Wen X, Yu M, Wu J, Bao X, Wang X, Gong P, He J, Chen X, Xiong X
OpenAccess logo Nat Commun 15 4620 (2024)
PMID: 38816392
Related structures: EMD-39848

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-244765 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 622 amino acids
Theoretical weight: 71.62 KDa
Source organism: Thogoto virus (isolate SiAr 126)
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P27194 (Residues: 1-622; Coverage: 100%)
Gene name: Segment 3
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 710 amino acids
Theoretical weight: 81.43 KDa
Source organism: Thogoto virus (isolate SiAr 126)
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O41353 (Residues: 1-710; Coverage: 100%)
Gene name: Segment 2
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: C
Molecule details ›
Chain: C
Length: 827 amino acids
Theoretical weight: 94.42 KDa
Source organism: Thogoto virus (isolate SiAr 126)
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9YNA4 (Residues: 1-769; Coverage: 100%)
Gene name: Segment 1
Sequence domains:
RNA (5'-R(*AP*GP*AP*GP*AP*AP*AP*UP*CP*AP*AP*GP*GP*CP*AP*GP*UP*U)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.84 KDa
RNA (5'-R(*GP*AP*CP*UP*GP*CP*CP*UP*GP*UP*UP*UP*UP*UP*GP*CP*U)-3') Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.34 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.16Å
Relevant EMDB volumes: EMD-39848
Expression system: Spodoptera frugiperda

Quick links