› 8z99

Electron Microscopy
3.2Å resolution

Cryo-EM structure of NTR-bound type VII CRISPR-Cas complex at substrate-engaged state +I

Released:
DOI: 10.2210/pdb8z99/pdb
PDB entry 8z99 coloured by chain, front view.
PDB entry 8z99 coloured by chain, side view.
PDB entry 8z99 coloured by chain, top view.
Source organism: metagenome
Primary publication:
Structural basis for the activity of the type VII CRISPR-Cas system.
Yang J, Li X, He Q, Wang X, Tang J, Wang T, Zhang Y, Yu F, Zhang S, Liu Z, Zhang L, Liao F, Yin H, Zhao H, Deng Z, Zhang H
Nature (2024)
PMID: 39143216
Related structures: EMD-39857

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-250659 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
a protein Chains: A, B, C, D, E, F, J, P
Molecule details ›
Chains: A, B, C, D, E, F, J, P
Length: 200 amino acids
Theoretical weight: 22.26 KDa
Source organism: metagenome
Expression system: Escherichia coli
a protein Chain: G
Molecule details ›
Chain: G
Length: 240 amino acids
Theoretical weight: 27.14 KDa
Source organism: metagenome
Expression system: Escherichia coli
a protein Chains: H, I, K, L
Molecule details ›
Chains: H, I, K, L
Length: 609 amino acids
Theoretical weight: 70.47 KDa
Source organism: metagenome
Expression system: Escherichia coli
RNA (54-MER) Chain: M
Molecule details ›
Chain: M
Length: 60 nucleotides
Theoretical weight: 19.14 KDa
RNA (49-MER) Chain: N
Molecule details ›
Chain: N
Length: 60 nucleotides
Theoretical weight: 19.35 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 9 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-39857
Expression system: Escherichia coli

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