› 8zfw

X-ray diffraction
2.18Å resolution

norbelladine 4'-O-methyltransferase S52T Y186F complexed with Mg and SAH

Released:
DOI: 10.2210/pdb8zfw/pdb
PDB entry 8zfw coloured by chain, front view.
PDB entry 8zfw coloured by chain, side view.
PDB entry 8zfw coloured by chain, top view.
Source organism: Narcissus aff. pseudonarcissus MK-2014
Primary publication:
Structure-Based Catalytic Mechanism of Amaryllidaceae O-Methyltransferases
Hnin SYY, Nakashima Y, Kodama T, Morita H
ACS Catalysis 11865-11880 (2024)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + norbelladine = S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-492602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Norbelladine 4'-O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.18 KDa
Source organism: Narcissus aff. pseudonarcissus MK-2014
Expression system: Escherichia coli M15
UniProt:
  • Canonical: A0A077EWA5 (Residues: 1-239; Coverage: 100%)
Gene name: N4OMT
Sequence domains: O-methyltransferase

Ligands and Environments

4 bound ligands:
Ligand GOL 2 x GOL
Ligand MG 2 x MG
Ligand SAH 2 x SAH
Ligand A1LU2 1 x A1LU2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P21212
Unit cell:
a: 80.501Å b: 116.587Å c: 50.297Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.214
Expression system: Escherichia coli M15

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