› 8zjd

Electron Microscopy
3.06Å resolution

Cryo-EM structure of kisspeptin receptor bound to KP-10

Released:
DOI: 10.2210/pdb8zjd/pdb
PDB entry 8zjd coloured by chain, front view.
PDB entry 8zjd coloured by chain, side view.
PDB entry 8zjd coloured by chain, top view.
Source organisms:
Entry authors: Shen S, Liu H, Xu HE
Related structures: EMD-60141

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-247387 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
KiSS-1 receptor Chain: R
Molecule details ›
Chain: R
Length: 512 amino acids
Theoretical weight: 55.95 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q969F8 (Residues: 2-355; Coverage: 89%)
Gene names: AXOR12, GPR54, KISS1R
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Kisspeptin-10 Chain: L
Molecule details ›
Chain: L
Length: 11 amino acids
Theoretical weight: 1.38 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: Q15726 (Residues: 112-121; Coverage: 8%)
Gene names: KISS1, PP5098
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 247 amino acids
Theoretical weight: 26.28 KDa
Source organism: Mus sp.
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(q) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 41.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P50148 (Residues: 7-7, 37-359; Coverage: 90%)
  • Canonical: P63096 (Residues: 2-28; Coverage: 8%)
Gene names: GAQ, GNAI1, GNAQ
Sequence domains: G-protein alpha subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.06Å
Relevant EMDB volumes: EMD-60141
Expression systems:
  • Trichoplusia ni
  • Not provided
  • Spodoptera frugiperda

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