› 8zn2

X-ray diffraction
2.65Å resolution

Structure of Phosphopantetheine adenylyltransferase (PPAT) from Enterobacter sp. with the expression tag bound in the substrate binding site of a neighbouring molecule at 2.65 A resolution.

Released:
DOI: 10.2210/pdb8zn2/pdb
PDB entry 8zn2 coloured by chain, front view.
PDB entry 8zn2 coloured by chain, side view.
PDB entry 8zn2 coloured by chain, top view.
Source organism: Enterobacter sp. 638
Entry authors: Ahmad N, Sharma P, Bhushan A, Sharma S, Singh TP

Function and Biology Details

Reaction catalysed: 
ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphopantetheine adenylyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 173 amino acids
Theoretical weight: 19.18 KDa
Source organism: Enterobacter sp. 638
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4W515 (Residues: 1-159; Coverage: 100%)
Gene names: Ent638_0105, coaD
Sequence domains: Cytidylyltransferase-like

Ligands and Environments

3 bound ligands:
Ligand PAE 6 x PAE
Ligand GOL 2 x GOL
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 137.929Å b: 78.643Å c: 107.005Å
α: 90° β: 93.117° γ: 90°
R-values:
R R work R free
0.173 0.17 0.234
Expression system: Escherichia coli BL21(DE3)

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