8zq9

Electron Microscopy
2.87Å resolution

Cryo-EM structure of the Cas9d-sgRNA complex

Released:
Source organism: metagenome
Entry authors: Zhang H, Li X, Liu Z
Related structures: EMD-60379

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-284974 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
a protein Chain: B
Molecule details ›
Chain: B
Length: 747 amino acids
Theoretical weight: 86.7 KDa
Source organism: metagenome
Expression system: Escherichia coli
RNA (128-MER) Chain: A
Molecule details ›
Chain: A
Length: 159 nucleotides
Theoretical weight: 51.13 KDa
Source organism: metagenome
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.87Å
Relevant EMDB volumes: EMD-60379
Expression system: Escherichia coli