8zqa

X-ray diffraction
2.91Å resolution

Crystal structure of 1,4-alpha-glucan branching protein from Rhodothermus profundi

Released:
Model geometry
Fit model/data
Source organism: Rhodothermus profundi
Entry authors: Li Q, Zong ZY, Liu WD

Function and Biology Details

Reaction catalysed:
Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo 40-mer (preferred)
PDBe Complex ID:
PDB-CPX-268957 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1,4-alpha-glucan branching enzyme GlgB Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 620 amino acids
Theoretical weight: 72.38 KDa
Source organism: Rhodothermus profundi
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A1M6PD84 (Residues: 1-620; Coverage: 100%)
Gene names: SAMN04488087_0136, glgB
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL10U2
Spacegroup: P1
Unit cell:
a: 100.302Å b: 112.266Å c: 166.26Å
α: 73.59° β: 73.77° γ: 65.25°
R-values:
R R work R free
0.2 0.197 0.256
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'