› 8zqe

Electron Microscopy
2.9Å resolution

Cryo-EM structure of the GPR15L(C11)-bound GPR15 complex

Released:
DOI: 10.2210/pdb8zqe/pdb
PDB entry 8zqe coloured by chain, front view.
PDB entry 8zqe coloured by chain, side view.
PDB entry 8zqe coloured by chain, top view.
Source organisms:
Primary publication:
Molecular recognition of the atypical chemokine-like peptide GPR15L by its cognate receptor GPR15.
Zhang Z, Zheng Y, Xu L, Yue Y, Xu K, Li F, Xu F
OpenAccess logo Cell Discov 10 69 (2024)
PMID: 38918398
Related structures: EMD-60384

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-272577 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.47 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 97 amino acids
Theoretical weight: 10.51 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 279 amino acids
Theoretical weight: 29.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
G-protein coupled receptor 15; Maltose/maltodextrin-binding periplasmic protein; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 881 amino acids
Theoretical weight: 98.62 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 3-18, 19-127; Coverage: 99%)
  • Canonical: P49685 (Residues: 1-360; Coverage: 100%)
  • Canonical: P0AEX9 (Residues: 25-392; Coverage: 99%)
Gene names: GPR15, JW3994, b4034, cybC, malE
Sequence domains:
Protein GPR15LG Chain: L
Molecule details ›
Chain: L
Length: 10 amino acids
Theoretical weight: 1.07 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q6UWK7 (Residues: 72-81; Coverage: 18%)
Gene names: C10orf99, GPR15L, GPR15LG, UNQ1833/PRO3446

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-60384
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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