› 8zrk

Electron Microscopy
2.82Å resolution

Cryo-EM structure of GPR119-Gs Complex with small molecule agonist GSK-1292263

Released:
DOI: 10.2210/pdb8zrk/pdb
PDB entry 8zrk coloured by chain, front view.
PDB entry 8zrk coloured by chain, side view.
PDB entry 8zrk coloured by chain, top view.
Source organisms:
Entry authors: Wong TS, Xiong TT, Zeng ZC, Gan SY, Qiu C, Du Y
Related structures: EMD-60399

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-246061 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 384 amino acids
Theoretical weight: 42.58 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 157 amino acids
Theoretical weight: 17.06 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Glucose-dependent insulinotropic receptor Chain: R
Molecule details ›
Chain: R
Length: 369 amino acids
Theoretical weight: 40.76 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TDV5 (Residues: 1-335; Coverage: 100%)
Gene name: GPR119
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

1 bound ligand:
Ligand A1D8Y 1 x A1D8Y
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.82Å
Relevant EMDB volumes: EMD-60399
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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