› 8zuh

X-ray diffraction
3.2Å resolution

Crystal structure of bovine Fbs2/Skp1/Man3GlcNAc2 complex

Released:
DOI: 10.2210/pdb8zuh/pdb
PDB entry 8zuh coloured by chain, front view.
PDB entry 8zuh coloured by chain, side view.
PDB entry 8zuh coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural basis of sugar recognition by SCFFBS2 ubiquitin ligase involved in NGLY1 deficiency.
Satoh T, Yagi-Utsumi M, Ishii N, Mizushima T, Yagi H, Kato R, Tachida Y, Tateno H, Matsuo I, Kato K, Suzuki T, Yoshida Y
FEBS Lett (2024)
PMID: 39171510

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-278795 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
F-box only protein 6 Chains: A, C
Molecule details ›
Chains: A, C
Length: 265 amino acids
Theoretical weight: 30.81 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3SX24 (Residues: 1-265; Coverage: 100%)
Gene names: FBS2, FBX6, FBXO6
Sequence domains:
S-phase kinase-associated protein 1 Chain: B
Molecule details ›
Chain: B
Length: 166 amino acids
Theoretical weight: 18.97 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3ZCF3 (Residues: 1-163; Coverage: 100%)
Gene names: SKP1, SKP1A
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P41
Unit cell:
a: 115.72Å b: 115.72Å c: 110.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.247
Expression system: Escherichia coli BL21(DE3)

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