› 8zut

X-ray diffraction
1.48Å resolution

Crystal structure of the F99S/M153T/V163A variant of GFP at pH 8.5

Released:
DOI: 10.2210/pdb8zut/pdb
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PDB entry 8zut coloured by chain, front view.
PDB entry 8zut coloured by chain, side view.
PDB entry 8zut coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural characterization of green fluorescent protein in the I-state.
Takeda R, Tsutsumi E, Okatsu K, Fukai S, Takeda K
Sci Rep 14 22832 (2024)
PMID: 39353998

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 25.84 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-231; Coverage: 97%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand GYS 1 x GYS

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 50.536Å b: 62.347Å c: 67.758Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.197
Expression system: Escherichia coli BL21(DE3)

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