› 9c7v

Electron Microscopy
6.6Å resolution

Structure of the human BOS:human EMC complex in GDN

Released:
DOI: 10.2210/pdb9c7v/pdb
PDB entry 9c7v coloured by chain, front view.
PDB entry 9c7v coloured by chain, side view.
PDB entry 9c7v coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins.
Page KR, Nguyen VN, Pleiner T, Tomaleri GP, Wang ML, Guna A, Hazu M, Wang TY, Chou TF, Voorhees RM
Mol Cell (2024)
PMID: 39173640
Related structures: EMD-45295

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-520019 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (13 distinct):
ER membrane protein complex subunit 10 Chain: 10
Molecule details ›
Chain: 10
Length: 262 amino acids
Theoretical weight: 27.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q5UCC4 (Residues: 1-262; Coverage: 100%)
Gene names: C19orf63, EMC10, INM02, UNQ764/PRO1556
Sequence domains: ER membrane protein complex subunit 10
ER membrane protein complex subunit 1 Chain: 1
Molecule details ›
Chain: 1
Length: 993 amino acids
Theoretical weight: 111.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q8N766 (Residues: 1-993; Coverage: 100%)
Gene names: EMC1, KIAA0090, PSEC0263
Sequence domains:
ER membrane protein complex subunit 2 Chain: 2
Molecule details ›
Chain: 2
Length: 297 amino acids
Theoretical weight: 34.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15006 (Residues: 1-297; Coverage: 100%)
Gene names: EMC2, KIAA0103, TTC35
Sequence domains: EMC2 TPR-like repeat domain
ER membrane protein complex subunit 3 Chain: 3
Molecule details ›
Chain: 3
Length: 261 amino acids
Theoretical weight: 29.98 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9P0I2 (Residues: 1-261; Coverage: 100%)
Gene names: EMC3, TMEM111
Sequence domains: Integral membrane protein EMC3/TMCO1-like
ER membrane protein complex subunit 4 Chain: 4
Molecule details ›
Chain: 4
Length: 183 amino acids
Theoretical weight: 20.1 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q5J8M3 (Residues: 1-183; Coverage: 100%)
Gene names: EMC4, HSPC184, PIG17, TMEM85
Sequence domains: Protein of unknown function (DUF1077)
ER membrane protein complex subunit 5 Chain: 5
Molecule details ›
Chain: 5
Length: 131 amino acids
Theoretical weight: 14.71 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q8N4V1 (Residues: 1-131; Coverage: 100%)
Gene names: EMC5, MMGT1, TMEM32
Sequence domains: Membrane magnesium transporter
ER membrane protein complex subunit 6 Chain: 6
Molecule details ›
Chain: 6
Length: 110 amino acids
Theoretical weight: 12.03 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9BV81 (Residues: 1-110; Coverage: 100%)
Gene names: EMC6, TMEM93
Sequence domains: EMC6
Endoplasmic reticulum membrane protein complex subunit 7 Chain: 7
Molecule details ›
Chain: 7
Length: 242 amino acids
Theoretical weight: 26.5 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NPA0 (Residues: 1-242; Coverage: 100%)
Gene names: C11orf3, C15orf24, EMC7, HT022, UNQ905/PRO1926
Sequence domains: ER membrane protein complex subunit 7, beta-sandwich domain
ER membrane protein complex subunit 8 Chain: 8
Molecule details ›
Chain: 8
Length: 210 amino acids
Theoretical weight: 23.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O43402 (Residues: 1-210; Coverage: 100%)
Gene names: C16orf2, C16orf4, COX4AL, COX4NB, EMC8, FAM158B, NOC4
Sequence domains: Uncharacterised protein family (UPF0172)
BOS complex subunit NCLN Chain: A
Molecule details ›
Chain: A
Length: 563 amino acids
Theoretical weight: 63.05 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q969V3 (Residues: 1-563; Coverage: 100%)
Gene name: NCLN
Sequence domains: Peptidase family M28
BOS complex subunit NOMO2 Chain: B
Molecule details ›
Chain: B
Length: 1267 amino acids
Theoretical weight: 139.58 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q5JPE7 (Residues: 1-1267; Coverage: 100%)
Gene name: NOMO2
Sequence domains:
BOS complex subunit TMEM147 Chain: C
Molecule details ›
Chain: C
Length: 224 amino acids
Theoretical weight: 25.28 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BVK8 (Residues: 1-224; Coverage: 100%)
Gene name: TMEM147
Sequence domains: Predicted membrane protein (DUF2053)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.6Å
Relevant EMDB volumes: EMD-45295
Expression system: Homo sapiens

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