› 9fa3

X-ray diffraction
1.36Å resolution

Function and Biology Details

Reactions catalysed: 
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysosomal acid glucosylceramidase Chain: A
Molecule details ›
Chain: A
Length: 546 amino acids
Theoretical weight: 61.16 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04062 (Residues: 1-536; Coverage: 100%)
Gene names: GBA, GBA1, GC, GLUC
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 2 x NAG
Ligand K 2 x K
Ligand SO4 1 x SO4
Ligand A1IBC 1 x A1IBC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 52.806Å b: 74.744Å c: 68.419Å
α: 90° β: 102.56° γ: 90°
R-values:
R R work R free
0.174 0.173 0.205
Expression system: Spodoptera frugiperda

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