› 9fd5

X-ray diffraction
1.31Å resolution

flavin reductase ThdF in complex with two bound FADs in space group P21

Released:
DOI: 10.2210/pdb9fd5/pdb
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PDB entry 9fd5 coloured by chain, front view.
PDB entry 9fd5 coloured by chain, side view.
PDB entry 9fd5 coloured by chain, top view.
Source organism: Streptomyces albogriseolus
Primary publication:
The NADH-dependent flavin reductase ThdF follows an ordered sequential mechanism though crystal structures reveal two FAD molecules in the active site.
Horstmeier HJ, Bork S, Nagel MF, Keller W, Sproß J, Diepold N, Ruppel M, Kottke T, Niemann HH
J Biol Chem 108128 (2024)
PMID: 39725031

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-283019 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin reductase like domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 195 amino acids
Theoretical weight: 20.33 KDa
Source organism: Streptomyces albogriseolus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1B1V585 (Residues: 3-193; Coverage: 99%)
Gene name: thdF
Sequence domains: Flavin reductase like domain

Ligands and Environments

1 bound ligand:
Ligand FAD 8 x FAD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P21
Unit cell:
a: 59.15Å b: 77.37Å c: 75.49Å
α: 90° β: 111.827° γ: 90°
R-values:
R R work R free
0.117 0.115 0.153
Expression system: Escherichia coli BL21(DE3)

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