› 9h9e

Electron Microscopy
3.6Å resolution

Cryo-EM structure of the human GABAA receptor alpha1 subunit in complex with the assembly factor NACHO/TMEM35A

Released:
DOI: 10.2210/pdb9h9e/pdb
PDB entry 9h9e coloured by chain, front view.
PDB entry 9h9e coloured by chain, side view.
PDB entry 9h9e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of NACHO-mediated assembly of pentameric ligand-gated ion channels.
Hooda Y, Sente A, Judy RM, Smalinskaitė L, Peak-Chew SY, Naydenova K, Malinauskas T, Hardwick SW, Chirgadze DY, Aricescu AR, Hegde RS
bioRxiv (2024)
PMID: 39553992
Related structures: EMD-51963

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-281546 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Gamma-aminobutyric acid receptor subunit alpha-1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 484 amino acids
Theoretical weight: 54.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P14867 (Residues: 37-456; Coverage: 98%)
Gene name: GABRA1
Sequence domains:
Novel acetylcholine receptor chaperone Chains: B, C
Molecule details ›
Chains: B, C
Length: 187 amino acids
Theoretical weight: 20.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q53FP2 (Residues: 1-167; Coverage: 100%)
Gene names: NACHO, TMEM35, TMEM35A

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand PX6 2 x PX6
Ligand PC7 2 x PC7
Ligand PGT 2 x PGT
Ligand CLR 2 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-51963
Expression system: Homo sapiens

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