› 9j4j

X-ray diffraction
2.8Å resolution

Crystal structure of GH9l Inulin fructotransferases(IFTase)incomplex with nystose(F3)

Released:
DOI: 10.2210/pdb9j4j/pdb
PDB entry 9j4j coloured by chain, front view.
PDB entry 9j4j coloured by chain, side view.
PDB entry 9j4j coloured by chain, top view.
Source organism: Paenarthrobacter aurescens
Primary publication:
Elucidation of the mechanism underlying the sequential catalysis of inulin by fructotransferase.
Chen G, Wang ZX, Yang Y, Li Y, Zhang T, Ouyang S, Zhang L, Chen Y, Ruan X, Miao M
Int J Biol Macromol 134446 (2024)
PMID: 39098696

Function and Biology Details

Reaction catalysed: 
Produces alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,3'-dianhydride (DFA III) by successively eliminating the diminishing (2->1)-beta-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-514675 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Periplasmic copper-binding protein NosD beta helix domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 404 amino acids
Theoretical weight: 43.21 KDa
Source organism: Paenarthrobacter aurescens
Expression system: Escherichia coli
UniProt:
  • Canonical: F8QV43 (Residues: 48-450; Coverage: 90%)
Gene name: ift
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: FRU
Ligand FRU 2 x FRU
Carbohydrate polymer : NEW Components: FRU, GLC
Ligand FRU 15 x FRU
Ligand GLC 5 x GLC
Carbohydrate polymer : NEW Components: FRU
Ligand FRU 3 x FRU
1 bound ligand:
Ligand FRU 1 x FRU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P65
Unit cell:
a: 167.642Å b: 167.642Å c: 85.297Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.158 0.208
Expression system: Escherichia coli

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