› 9jog

X-ray diffraction
1.4Å resolution

COMPLEX STRUCTURE OF ENDO-1,3-FUCANASE (FUN168D) WITH FUCOTETRAOSE FROM HOLOTHURIA TUBULOSA

Released:
DOI: 10.2210/pdb9jog/pdb
PDB entry 9jog coloured by chain, front view.
PDB entry 9jog coloured by chain, side view.
PDB entry 9jog coloured by chain, top view.
Source organism: Wenyingzhuangia fucanilytica
Entry authors: Chen GN, Chang YG

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-579915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
endo-1.3-fucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 423 amino acids
Theoretical weight: 48.01 KDa
Source organism: Wenyingzhuangia fucanilytica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1B1Y6G8 (Residues: 22-410; Coverage: 95%)
Gene name: AXE80_08865
Sequence domains: Hypothetical glycosyl hydrolase family 15

Ligands and Environments

Carbohydrate polymer : NEW Components: X6Y, FUC, X2Y
Ligand X6Y 4 x X6Y
Ligand FUC 2 x FUC
Ligand X2Y 2 x X2Y
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 54.741Å b: 138.39Å c: 62.866Å
α: 90° β: 113.66° γ: 90°
R-values:
R R work R free
0.14 0.14 0.155
Expression system: Escherichia coli BL21(DE3)

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