› 9rnt

X-ray diffraction
1.5Å resolution

RIBONUCLEASE T1 WITH FREE RECOGNITION AND CATALYTIC SITE: CRYSTAL STRUCTURE ANALYSIS AT 1.5 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb9rnt/pdb
PDB entry 9rnt coloured by chain, front view.
PDB entry 9rnt coloured by chain, side view.
PDB entry 9rnt coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Ribonuclease T1 with free recognition and catalytic site: crystal structure analysis at 1.5 A resolution.
Martinez-Oyanedel J, Choe HW, Heinemann U, Saenger W
J Mol Biol 222 335-52 (1991)
PMID: 1960730

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Aspergillus oryzae
Expression system: Not provided
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 48.73Å b: 46.39Å c: 41.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 not available not available
Expression system: Not provided

Quick links

Citations

3 review citations

The ancient regulatory-protein family of WD-repeat proteins.
Neer et al. (1994)
2 more

22 mentions without citation

Fungal Ribotoxins: A Review of Potential Biotechnological Applications.
Olombrada et al. (2017)
21 more