› 9asl

Electron Microscopy
3.5Å resolution

Human DNA polymerase theta helicase domain tetramer, apo-form

Released:
DOI: 10.2210/pdb9asl/pdb
PDB entry 9asl coloured by chain, front view.
PDB entry 9asl coloured by chain, side view.
PDB entry 9asl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for Polθ-Helicase DNA Binding and Microhomology-Mediated End-Joining.
Ito F, Li Z, Minakhin L, Khant HA, Pomerantz RT, Chen XS
bioRxiv (2024)
PMID: 38895274
Related structures: EMD-43818

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130954 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase theta Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 894 amino acids
Theoretical weight: 99.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75417 (Residues: 1-894; Coverage: 35%)
Gene names: POLH, POLQ
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-43818
Expression system: Escherichia coli

Quick links