› 9au3

X-ray diffraction
1.35Å resolution

Crystal structure of GenB2 in complex with G418

Released:
DOI: 10.2210/pdb9au3/pdb
PDB entry 9au3 coloured by chain, front view.
PDB entry 9au3 coloured by chain, side view.
PDB entry 9au3 coloured by chain, top view.
Source organism: Micromonospora echinospora
Primary publication:
Structural and Functional Basis of GenB2 Isomerase Activity from Gentamicin Biosynthesis.
Oliveira GS, Dos S Bury P, Huang F, Li Y, Araújo NC, Zhou J, Sun Y, Leeper FJ, Leadlay PF, Dias MVB
ACS Chem Biol (2024)
PMID: 39207862

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative gentamicin aminotransferase II Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 44.63 KDa
Source organism: Micromonospora echinospora
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q70KE6 (Residues: 1-414; Coverage: 100%)
Gene names: gacE, genB2, gntL
Sequence domains: Aminotransferase class-III

Ligands and Environments

3 bound ligands:
Ligand PMP 1 x PMP
Ligand GET 1 x GET
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS SIRIUS BEAMLINE MANACA
Spacegroup: C2221
Unit cell:
a: 75.142Å b: 103.052Å c: 104.133Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.14 0.166
Expression system: Escherichia coli BL21(DE3)

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