› 9azp

Electron Microscopy
3.79Å resolution

INF2 at the Barbed End of F-Actin with Incoming Profilin-Actin

Released:
DOI: 10.2210/pdb9azp/pdb
PDB entry 9azp coloured by chain, front view.
PDB entry 9azp coloured by chain, side view.
PDB entry 9azp coloured by chain, top view.
Source organisms:
Primary publication:
Mechanisms of actin filament severing and elongation by formins.
Palmer NJ, Barrie KR, Dominguez R
Nature (2024)
PMID: 38843827
Related structures: EMD-44018

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-272396 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E, F, I
Molecule details ›
Chains: A, B, C, D, E, F, I
Length: 371 amino acids
Theoretical weight: 41.39 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 7-377; Coverage: 98%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Inverted formin-2 Chains: G, H
Molecule details ›
Chains: G, H
Length: 1249 amino acids
Theoretical weight: 135.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27J81 (Residues: 1-1249; Coverage: 100%)
Gene names: C14orf151, C14orf173, INF2
Sequence domains:
Profilin-1 Chain: J
Molecule details ›
Chain: J
Length: 139 amino acids
Theoretical weight: 14.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07737 (Residues: 2-140; Coverage: 99%)
Gene name: PFN1
Sequence domains: Profilin

Ligands and Environments

3 bound ligands:
Ligand ADP 6 x ADP
Ligand MG 7 x MG
Ligand ATP 1 x ATP
1 modified residue:
Ligand HIC 7 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 3.79Å
Relevant EMDB volumes: EMD-44018
Expression system: Escherichia coli

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