› 9b03

Electron Microscopy
2.95Å resolution

INF2 in the Middle of F-Actin (Up state)

Released:
DOI: 10.2210/pdb9b03/pdb
PDB entry 9b03 coloured by chain, front view.
PDB entry 9b03 coloured by chain, side view.
PDB entry 9b03 coloured by chain, top view.
Source organisms:
Primary publication:
Mechanisms of actin filament severing and elongation by formins.
Palmer NJ, Barrie KR, Dominguez R
Nature (2024)
PMID: 38843827
Related structures: EMD-44026

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274136 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 371 amino acids
Theoretical weight: 41.39 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 7-377; Coverage: 98%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Inverted formin-2 Chains: G, H
Molecule details ›
Chains: G, H
Length: 437 amino acids
Theoretical weight: 49.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27J81 (Residues: 551-987; Coverage: 35%)
Gene names: C14orf151, C14orf173, INF2
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 6 x ADP
Ligand MG 6 x MG
1 modified residue:
Ligand HIC 6 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 2.95Å
Relevant EMDB volumes: EMD-44026
Expression system: Escherichia coli

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