› 9b4s

X-ray diffraction
3.1Å resolution

Crystal structure of the RRAS2-p110alpha complex

Released:
DOI: 10.2210/pdb9b4s/pdb
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PDB entry 9b4s coloured by chain, front view.
PDB entry 9b4s coloured by chain, side view.
PDB entry 9b4s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into isoform-specific RAS-PI3Kα interactions and the role of RAS in PI3Kα activation.
Czyzyk D, Yan W, Messing S, Gillette W, Tsuji T, Yamaguchi M, Furuzono S, Turner DM, Esposito D, Nissley DV, McCormick F, Simanshu DK
Nat Commun 16 525 (2025)
PMID: 39788953

Function and Biology Details

Reactions catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-284253 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 965 amino acids
Theoretical weight: 112.03 KDa
Source organism: Homo sapiens
Expression system: Trichoplacea
UniProt:
  • Canonical: P42336 (Residues: 105-1068; Coverage: 90%)
Gene name: PIK3CA
Sequence domains:
Ras-related protein R-Ras2 Chain: B
Molecule details ›
Chain: B
Length: 180 amino acids
Theoretical weight: 20.56 KDa
Source organism: Homo sapiens
Expression system: Trichoplacea
UniProt:
  • Canonical: P62070 (Residues: 1-179; Coverage: 88%)
  • Best match: P62070-2 (Residues: 1-102)
Gene names: RRAS2, TC21
Sequence domains: Ras family

Ligands and Environments

3 bound ligands:
Ligand 5H5 1 x 5H5
Ligand GNP 1 x GNP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P42212
Unit cell:
a: 181.488Å b: 181.488Å c: 96.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.272
Expression system: Trichoplacea

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