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Ubiquitin E1-Ub-E2 tetrahedral transthiolation intermediate mimic (doubly Ub-loaded) - Ub(T) class 10 map and model from cluster 5 (Ub(A)-AMP)

Source organism: Schizosaccharomyces pombe 972h-
Biochemical function: ATP binding
Biological process: protein ubiquitination
Cellular component: cytosol

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EC 2.3.2.23: E2 ubiquitin-conjugating enzyme

Reaction catalysed:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Systematic name:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine:[E2 ubiquitin-conjugating enzyme] ubiquitinyl transferase
Alternative Name(s):
  • UBC
  • Ubiquitin-carrier-protein E2
  • Ubiquitin-conjugating enzyme E2

EC 6.2.1.45: E1 ubiquitin-activating enzyme

Reaction catalysed:
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
Systematic name:
Ubiquitin:[E1 ubiquitin-activating enzyme] ligase (AMP-forming)
Alternative Name(s):
  • E1
  • Ubiquitin activating enzyme
  • Ubiquitin-activating enzyme E1

GO terms

Sequence families

Pfam Protein families (Pfam)
PF00240
Domain description: Ubiquitin family
Occurring in:
  1. Ubiquitin
  2. Ubiquitin
The deposited structure of PDB entry 9b5l contains 1 copy of Pfam domain PF00240 (Ubiquitin family) in Ubiquitin. Showing 1 copy in chain B.
The deposited structure of PDB entry 9b5l contains 1 copy of Pfam domain PF00240 (Ubiquitin family) in Ubiquitin. Showing 1 copy in chain D.

PF00899
Domain description: ThiF family
Occurring in:
  1. Ubiquitin-activating enzyme E1 1
The deposited structure of PDB entry 9b5l contains 2 copies of Pfam domain PF00899 (ThiF family) in Ubiquitin-activating enzyme E1 1. Showing 2 copies in chain A.

PF00179
Domain description: Ubiquitin-conjugating enzyme
Occurring in:
  1. Ubiquitin-conjugating enzyme E2 4
The deposited structure of PDB entry 9b5l contains 1 copy of Pfam domain PF00179 (Ubiquitin-conjugating enzyme) in Ubiquitin-conjugating enzyme E2 4. Showing 1 copy in chain C.

PF09358
Domain description: Ubiquitin fold domain
Occurring in:
  1. Ubiquitin-activating enzyme E1 1
The deposited structure of PDB entry 9b5l contains 1 copy of Pfam domain PF09358 (Ubiquitin fold domain) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain A.

PF10585
Domain description: Ubiquitin-activating enzyme, SCCH domain
Occurring in:
  1. Ubiquitin-activating enzyme E1 1
The deposited structure of PDB entry 9b5l contains 1 copy of Pfam domain PF10585 (Ubiquitin-activating enzyme, SCCH domain) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain A.

PF16191
Domain description: Ubiquitin-activating enzyme E1 four-helix bundle
Occurring in:
  1. Ubiquitin-activating enzyme E1 1
The deposited structure of PDB entry 9b5l contains 1 copy of Pfam domain PF16191 (Ubiquitin-activating enzyme E1 four-helix bundle) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain A.

PF16190
Domain description: Ubiquitin-activating enzyme E1 FCCH domain
Occurring in:
  1. Ubiquitin-activating enzyme E1 1
The deposited structure of PDB entry 9b5l contains 1 copy of Pfam domain PF16190 (Ubiquitin-activating enzyme E1 FCCH domain) in Ubiquitin-activating enzyme E1 1. Showing 1 copy in chain A.

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