› 9b70

Electron Microscopy
2.88Å resolution

Cryo-EM structure of MraY in complex with analogue 2

Released:
DOI: 10.2210/pdb9b70/pdb
PDB entry 9b70 coloured by chain, front view.
PDB entry 9b70 coloured by chain, side view.
PDB entry 9b70 coloured by chain, top view.
Source organisms:
Primary publication:
Development of a natural product optimization strategy for inhibitors against MraY, a promising antibacterial target.
Yamamoto K, Sato T, Hao A, Asao K, Kaguchi R, Kusaka S, Ruddarraju RR, Kazamori D, Seo K, Takahashi S, Horiuchi M, Yokota SI, Lee SY, Ichikawa S
OpenAccess logo Nat Commun 15 5085 (2024)
PMID: 38877016
Related structures: EMD-44293

Function and Biology Details

Reaction catalysed: 
UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271809 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phospho-N-acetylmuramoyl-pentapeptide-transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 40.95 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66465 (Residues: 1-359; Coverage: 100%)
Gene names: aq_053, mraY
Sequence domains:
MraYAA nanobody Chains: G, H
Molecule details ›
Chains: G, H
Length: 137 amino acids
Theoretical weight: 15.06 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand A1AI1 2 x A1AI1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.88Å
Relevant EMDB volumes: EMD-44293
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links