› 9bc5

Electron Microscopy
5.32Å resolution

AAV-2 Rep68-AAVS1 heptameric complex

Released:
DOI: 10.2210/pdb9bc5/pdb
PDB entry 9bc5 coloured by chain, front view.
PDB entry 9bc5 coloured by chain, side view.
PDB entry 9bc5 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of AAV2 Rep68 bound to integration site AAVS1: insights into the mechanism of DNA melting.
Jaiswal R, Braud B, Hernandez-Ramirez KC, Santosh V, Washington A, Escalante CR
Nucleic Acids Res 53 (2025)
PMID: 39883011
Related structures: EMD-44424

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-285131 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protein Rep68 Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 491 amino acids
Theoretical weight: 55.97 KDa
Source organism: adeno-associated virus 2
Expression system: synthetic construct
UniProt:
  • Canonical: P03132 (Residues: 2-490; Coverage: 91%)
Gene name: Rep68
Sequence domains:
AAVS1 DNA (41-MER) Sense strand Chain: H
Molecule details ›
Chain: H
Length: 50 nucleotides
Theoretical weight: 15.63 KDa
Source organism: Homo sapiens
Expression system: Not provided
AAVS1 DNA (41-MER) ANTISENSE Chain: I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 5.32Å
Relevant EMDB volumes: EMD-44424
Expression systems:
  • synthetic construct
  • Not provided

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