Sample information
Author description:
Alkalihalobacillus halodurans (Aha) trp RNA binding attenuation protein (TRAP) mutant dTRAP with Trp
Source organism:
Expression system:
Escherichia coli BL21(DE3)
Validation information
Experimental information
Specimen preparation
| Buffer name | pH | Details |
|---|---|---|
| - | 8 | 20 mM HEPES, 200 mM NaCl, pH 8 |
Vitrification
| Cryogen | Temperature | Instrument | Humidity | Details |
|---|---|---|---|---|
| - | - | FEI VITROBOT MARK IV | 100 | lotted with a blot force of 1 for 4 seconds at 277.15K and 100% relative humidity before being plunged frozen into liquid ethane |
Refinement statistics
Structure solution method:
Electron Microscopy
Resolution:
3.6 Å
