› 9bkg

X-ray diffraction
1.57Å resolution

Crystal structure of selenomethionine labeled bovine trypsin mutant - S195A solved by Sulphur-SAD at 1.54A wavelength

Released:
DOI: 10.2210/pdb9bkg/pdb
PDB entry 9bkg coloured by chain, front view.
PDB entry 9bkg coloured by chain, side view.
PDB entry 9bkg coloured by chain, top view.
Source organism: Bos taurus
Entry authors: Zhou D, Chen L, Rose JP, Wang BC

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease 1 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.4 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002
Spacegroup: P3121
Unit cell:
a: 54.708Å b: 54.708Å c: 107.174Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.186 0.204
Expression system: Escherichia coli BL21(DE3)

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