› 9bkj

Electron Microscopy
2.59Å resolution

Cholecystokinin 1 receptor (CCK1R) Y140A mutant, Gq chimera (mGsqi) complex

Released:
DOI: 10.2210/pdb9bkj/pdb
PDB entry 9bkj coloured by chain, front view.
PDB entry 9bkj coloured by chain, side view.
PDB entry 9bkj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cholesterol-dependent dynamic changes in the conformation of the type 1 cholecystokinin receptor affect ligand binding and G protein coupling.
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Dong M, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
PLoS Biol 22 e3002673 (2024)
PMID: 39083706
Related structures: EMD-44642

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-138995 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.41 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 58 amino acids
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 5-62; Coverage: 82%)
Gene name: GNG2
Sequence domains: GGL domain
Cholecystokinin-8 Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 1.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P06307 (Residues: 96-103; Coverage: 8%)
Gene name: CCK
Cholecystokinin receptor type A Chain: R
Molecule details ›
Chain: R
Length: 427 amino acids
Theoretical weight: 47.66 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P32238 (Residues: 2-428; Coverage: 100%)
Gene names: CCKAR, CCKRA
Sequence domains:
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 29.14 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63096 (Residues: 2-57, 181-181, 229-242, 309-309, 319-319, 347-348; Coverage: 18%)
  • Canonical: P63092 (Residues: 205-251, 266-346, 348-357, 359-386, 389-394; Coverage: 44%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit

Ligands and Environments

1 bound ligand:
Ligand NH2 1 x NH2
1 modified residue:
Ligand TYS 1 x TYS

Experiments and Validation Details

Entry percentile scores
Resolution: 2.59Å
Relevant EMDB volumes: EMD-44642
Expression systems:
  • Trichoplusia ni
  • Not provided

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