› 9bp9

Electron Microscopy
3.21Å resolution

Human DNA polymerase theta helicase domain in complex with inhibitor AB25583, dimer form

Released:
DOI: 10.2210/pdb9bp9/pdb
PDB entry 9bp9 coloured by chain, front view.
PDB entry 9bp9 coloured by chain, side view.
PDB entry 9bp9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for a Polθ helicase small-molecule inhibitor revealed by cryo-EM.
Ito F, Li Z, Minakhin L, Chandramouly G, Tyagi M, Betsch R, Krais JJ, Taberi B, Vekariya U, Calbert M, Skorski T, Johnson N, Chen XS, Pomerantz RT
Nat Commun 15 7003 (2024)
PMID: 39143110
Related structures: EMD-44765

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243709 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase theta Chains: A, C
Molecule details ›
Chains: A, C
Length: 894 amino acids
Theoretical weight: 99.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75417 (Residues: 1-894; Coverage: 35%)
Gene names: POLH, POLQ
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand WCN 2 x WCN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.21Å
Relevant EMDB volumes: EMD-44765
Expression system: Escherichia coli

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