› 9byj

X-ray diffraction
1.8Å resolution

Crystal Structure of Hck in complex with the Src-family kinase inhibitor A-419259

Released:
DOI: 10.2210/pdb9byj/pdb
PDB entry 9byj coloured by chain, front view.
PDB entry 9byj coloured by chain, side view.
PDB entry 9byj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cocrystallization of the Src-Family Kinase Hck with the ATP-Site Inhibitor A-419259 Stabilizes an Extended Activation Loop Conformation.
Selzer AM, Alvarado JJ, Smithgall TE
Biochemistry (2024)
PMID: 39315638

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase HCK Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 52.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08631 (Residues: 81-526; Coverage: 85%)
Gene name: HCK
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand VSE 1 x VSE
Ligand EDO 6 x EDO
Ligand DMS 2 x DMS
Ligand SCN 15 x SCN
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 43.346Å b: 85.025Å c: 128.798Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.219
Expression system: Escherichia coli BL21(DE3)

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