› 9bzn

X-ray diffraction
1.05Å resolution

High resolution structure of class A Beta-lactamase from Bordetella bronchiseptica RB50

Released:
DOI: 10.2210/pdb9bzn/pdb
PDB entry 9bzn coloured by chain, front view.
PDB entry 9bzn coloured by chain, side view.
PDB entry 9bzn coloured by chain, top view.
Source organism: Bordetella bronchiseptica RB50
Entry authors: Maltseva N, Kim Y, Endres M, Joachimiak A, Center for Structural Biology of Infectious Diseases (CSBID)

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245591 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 28.91 KDa
Source organism: Bordetella bronchiseptica RB50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3LUA9 (Residues: 37-305; Coverage: 88%)
Gene name: BB2049
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FMT 1 x FMT
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 36.064Å b: 79.549Å c: 42.891Å
α: 90° β: 108.28° γ: 90°
R-values:
R R work R free
0.128 0.127 0.141
Expression system: Escherichia coli BL21(DE3)

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