› 9c0r

Electron Microscopy
2.8Å resolution

Carbon monoxide dehydrogenase (CODH) from Methanosarcina thermophila, specimen prepared on chameleon plunger

Released:
DOI: 10.2210/pdb9c0r/pdb
PDB entry 9c0r coloured by chain, front view.
PDB entry 9c0r coloured by chain, side view.
PDB entry 9c0r coloured by chain, top view.
Source organism: Methanosarcina thermophila
Primary publication:
Capturing a methanogenic carbon monoxide dehydrogenase/acetyl-CoA synthase complex via cryogenic electron microscopy.
Biester A, Grahame DA, Drennan CL
Proc Natl Acad Sci U S A 121 e2410995121 (2024)
PMID: 39361653
Related structures: EMD-45090

Function and Biology Details

Reaction catalysed: 
CO + H(2)O + 2 oxidized ferredoxin = CO(2) + 2 reduced ferredoxin + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-278829 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acetyl-CoA decarbonylase/synthase complex subunit alpha 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 803 amino acids
Theoretical weight: 87.86 KDa
Source organism: Methanosarcina thermophila
UniProt:
  • Canonical: Q9C4Z4 (Residues: 1-803; Coverage: 100%)
Gene name: cdhA2
Sequence domains:
Acetyl-CoA decarbonylase/synthase complex subunit epsilon 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 170 amino acids
Theoretical weight: 18.59 KDa
Source organism: Methanosarcina thermophila
UniProt:
  • Canonical: Q9C4Z3 (Residues: 1-170; Coverage: 100%)
Gene name: cdhB2
Sequence domains: CO dehydrogenase beta subunit/acetyl-CoA synthase epsilon subunit

Ligands and Environments

2 bound ligands:
Ligand SF4 7 x SF4
Ligand RQM 2 x RQM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-45090

Quick links