› 9c48

Electron Microscopy
2.4Å resolution

Cryo-EM structure of the full-length human P2X4 receptor in the ATP-bound desensitized state

Released:
DOI: 10.2210/pdb9c48/pdb
PDB entry 9c48 coloured by chain, front view.
PDB entry 9c48 coloured by chain, side view.
PDB entry 9c48 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human P2X4 receptor gating is modulated by a stable cytoplasmic cap and a unique allosteric pocket.
Shi H, Ditter IA, Oken AC, Mansoor SE
Sci Adv 11 eadr3315 (2025)
PMID: 39823330
Related structures: EMD-45177

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-284687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
P2X purinoceptor 4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 388 amino acids
Theoretical weight: 43.42 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q99571 (Residues: 1-388; Coverage: 100%)
Gene name: P2RX4
Sequence domains: ATP P2X receptor

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand ATP 3 x ATP
Ligand NAG 9 x NAG
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.4Å
Relevant EMDB volumes: EMD-45177
Expression system: Homo sapiens

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