› 9c5q

Electron Microscopy
3.1Å resolution

Human DNA polymerase theta helicase domain in microhomology annealed state 2, dimer form

Released:
DOI: 10.2210/pdb9c5q/pdb
PDB entry 9c5q coloured by chain, front view.
PDB entry 9c5q coloured by chain, side view.
PDB entry 9c5q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for Polθ-Helicase DNA Binding and Microhomology-Mediated End-Joining.
Ito F, Li Z, Minakhin L, Khant HA, Pomerantz RT, Chen XS
bioRxiv (2024)
PMID: 38895274
Related structures: EMD-45218

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246272 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA polymerase theta Chains: A, C
Molecule details ›
Chains: A, C
Length: 824 amino acids
Theoretical weight: 92.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75417 (Residues: 68-891; Coverage: 32%)
Gene names: POLH, POLQ
Sequence domains:
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*CP*CP*CP*GP*GP*G)-3') Chains: B, D
Molecule details ›
Chains: B, D
Length: 14 nucleotides
Theoretical weight: 4.24 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-45218
Expression systems:
  • Escherichia coli
  • Not provided

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