› 9cg4

Electron Microscopy
3.37Å resolution

Cryo-EM structure of FAN1, PCNA and DNA substrate with (CAG)2 extrusion in intermediate state

Released:
DOI: 10.2210/pdb9cg4/pdb
PDB entry 9cg4 coloured by chain, front view.
PDB entry 9cg4 coloured by chain, side view.
PDB entry 9cg4 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Fenglin L, Anna P
Related structures: EMD-45568

Function and Biology Details

Reaction catalysed: 
Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-285867 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Proliferating cell nuclear antigen Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 261 amino acids
Theoretical weight: 28.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Fanconi-associated nuclease 1 Chain: E
Molecule details ›
Chain: E
Length: 183 amino acids
Theoretical weight: 20.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y2M0 (Residues: 372-554; Coverage: 18%)
Gene names: FAN1, KIAA1018, MTMR15
Sequence domains:
DNA (5'-D(P*CP*GP*AP*AP*TP*TP*TP*CP*TP*AP*GP*AP*CP*TP*CP*GP*AP*GP*AP*TP*CP*AP*G)-3') Chain: A
DNA (5'-D(P*AP*TP*CP*TP*CP*GP*AP*GP*TP*CP*TP*AP*GP*AP*AP*AP*TP*TP*CP*G)-3') Chain: F

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.37Å
Relevant EMDB volumes: EMD-45568
Expression system: Escherichia coli BL21(DE3)

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