9cg7

Electron Microscopy
2.75Å resolution

70S initiation complex (tRNA-fMet M1 + AUG start codon)

Released:
Source organism: Escherichia coli
Related structures: EMD-45573

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 54-mer (preferred)
PDBe Complex ID:
PDB-CPX-251410 (preferred)
Entry contents:
49 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (54 distinct):
Large ribosomal subunit protein bL33 Chain: 0
Molecule details ›
Chain: 0
Length: 55 amino acids
Theoretical weight: 6.39 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: C3SME7 (Residues: 1-55; Coverage: 100%)
Gene names: A2J79_002320, A5U30_004136, ABE91_019545, ACU57_07205, AWP47_15985, B6R15_003224, B6R31_002865, BANRA_02297, BANRA_04677, BCB93_002421, BG944_002239, BGM66_003973, BGZ_04499, BK300_05155, BK383_09650, BKL28_001549, BMT50_10565, BMT91_13845, BRV02_004184, BTB68_002398, BTQ06_17850, BXT93_06220, BvCmsKKP061_03496, C0P57_003843, C1Q91_001907, C2M16_17175, C2R31_004081, C3F40_17705, C6669_09640, C719_001850, C7B02_19050, C9160_15120, C9194_14820, C9Z68_15420, CCS08_06865, CF22_002026, CG704_10010, CIG67_03795, CQ842_12590, CQ842_18775, CR538_00630, CTR35_001077, CV83915_02464, CWS33_14490, D3C88_33140, D3G36_09395, D4N09_11500, D9D43_20690, D9E49_10195, D9J61_14005, DD762_15060, DIV22_25130, DL968_03015, DNQ45_17840, DNX30_21910, DS732_26145, DTL43_17805, DU321_24275, E2863_04455, E4K51_19990, E5H86_15640, E6D34_14175, ECs4511, EIA08_13065, EIMP300_11190, EIZ93_22910, EN85_002943, EPS76_16410, EPS97_14120, EWK56_19235, EXX87_00245, ExPECSC038_00886, F7F11_22995, F7N46_06895, F9413_20155, F9461_04500, F9B07_09850, FGAF848_22460, FIJ20_14735, FJQ40_22750, FKO60_20795, FOI11_017545, FOI11_05635, FPI65_22380, FPS11_15665, FTV93_19030, FVB16_08575, FWK02_20790, FZU14_11310, G3V95_06555, G3W53_09755, G4A38_07645, G4A47_07035, G5603_14155, GAI89_21025, GAJ12_25750, GGB84_002816, GKF66_06090, GNW61_15010, GOP25_22240, GP711_14020, GP965_18420, GP975_06785, GQE64_12875, GQE86_16775, GQM04_18880, GQM13_19245, GQM21_20675, GQN24_12050, GRW05_17070, GRW24_04500, GTP92_21000, GUC01_19125, HEP30_004320, HEP34_000581, HHH44_001955, HI055_001939, HIE29_002315, HJQ60_004388, HKA49_003561, HLX92_21165, HLZ39_14555, HLZ50_07765, HMV95_17960, HV109_00345, HV209_24710, HVV39_10960, HVW04_15855, HVW43_16870, HVY77_00425, I6H02_13660, IFC14_004299, J0541_001519, J5U05_003670, J8F57_000299, JNA68_09080, JNP96_26980, NCTC10082_02483, NCTC10279_00984, NCTC10418_00180, NCTC10429_00616, NCTC10764_03481, NCTC10767_01151, NCTC10865_00201, NCTC10974_00127, NCTC11112_01599, NCTC11126_01709, NCTC11181_02394, NCTC11341_02337, NCTC7922_04520, NCTC7927_00189, NCTC7928_02970, NCTC8009_00971, NCTC8179_05536, NCTC8333_00121, NCTC8500_05792, NCTC8603_03615, NCTC8621_00171, NCTC8960_02723, NCTC8985_04527, NCTC9001_03782, NCTC9044_01835, NCTC9045_00169, NCTC9073_06389, NCTC9075_00237, NCTC9077_00137, NCTC9081_00502, NCTC9117_00296, NCTC9702_00106, NCTC9706_02375, NCTC9962_01072, NOI85_17610, NY836_11285, OFN31_09725, OGM49_24050, P6223_003111, PWL68_002984, Q2V20_23240, Q2V64_19740, QDW62_00450, QO046_15595, R8G00_15090, R8O40_002437, RZR61_11530, SAMEA3472044_00655, SAMEA3752557_01144, V9Z47_00575, WR15_15810, rpmG
Sequence domains: Ribosomal protein L33
Large ribosomal subunit protein bL34 Chain: 1
Molecule details ›
Chain: 1
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: B7MGC4 (Residues: 1-46; Coverage: 100%)
Gene names: ECS88_4126, rpmH
Sequence domains: Ribosomal protein L34
Large ribosomal subunit protein bL35 Chain: 2
Large ribosomal subunit protein bL36 Chain: 3
Large ribosomal subunit protein bL31 Chain: 4
Small ribosomal subunit protein uS2 Chain: B
Small ribosomal subunit protein uS3 Chain: C
Small ribosomal subunit protein uS4 Chain: D
Small ribosomal subunit protein uS5 Chain: E
Small ribosomal subunit protein bS6 Chain: F
Small ribosomal subunit protein uS7 Chain: G
Small ribosomal subunit protein uS8 Chain: H
Small ribosomal subunit protein uS9 Chain: I
Small ribosomal subunit protein uS10 Chain: J
Small ribosomal subunit protein uS11 Chain: K
Small ribosomal subunit protein uS12 Chain: L
Small ribosomal subunit protein uS13 Chain: M
Small ribosomal subunit protein uS14 Chain: N
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein bS16 Chain: P
Small ribosomal subunit protein uS17 Chain: Q
Small ribosomal subunit protein bS18 Chain: R
Small ribosomal subunit protein uS19 Chain: S
Small ribosomal subunit protein bS20 Chain: T
Small ribosomal subunit protein bS21 Chain: U
Large ribosomal subunit protein uL2 Chain: c
Large ribosomal subunit protein uL3 Chain: d
Large ribosomal subunit protein uL4 Chain: e
Large ribosomal subunit protein uL5 Chain: f
Large ribosomal subunit protein uL6 Chain: g
Large ribosomal subunit protein bL9 Chain: h
Large ribosomal subunit protein uL13 Chain: i
Large ribosomal subunit protein uL14 Chain: j
Large ribosomal subunit protein uL16 Chain: l
Large ribosomal subunit protein bL17 Chain: m
Large ribosomal subunit protein uL18 Chain: n
Large ribosomal subunit protein bL19 Chain: o
Large ribosomal subunit protein bL20 Chain: p
Large ribosomal subunit protein bL21 Chain: q
Large ribosomal subunit protein uL22 Chain: r
Large ribosomal subunit protein uL23 Chain: s
Large ribosomal subunit protein uL24 Chain: t
Large ribosomal subunit protein bL25 Chain: u
Large ribosomal subunit protein bL27 Chain: v
Large ribosomal subunit protein bL28 Chain: w
Large ribosomal subunit protein uL29 Chain: x
Large ribosomal subunit protein uL30 Chain: y
Large ribosomal subunit protein bL32 Chain: z
16S ribosomal RNA Chain: A
Messenger RNA Chain: X
P-site tRNA-fMet M1 Chain: Z
23S ribosomal RNA Chain: a
5S ribosomal RNA Chain: b

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.75Å
Relevant EMDB volumes: EMD-45573