› 9cg8

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE P285S VARIANT OF SERINE HYDROXYMETHYLTRANSFERASE 8 FROM SOYBEAN CULTIVAR FORREST

Released:
DOI: 10.2210/pdb9cg8/pdb
PDB entry 9cg8 coloured by chain, front view.
PDB entry 9cg8 coloured by chain, side view.
PDB entry 9cg8 coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Key structural role of a conserved cis-proline revealed by the P285S variant of soybean serine hydroxymethyltransferase 8.
Samarakoon V, Owuocha LF, Hammond J, Mitchum MG, Beamer L
Biochem J (2024)
PMID: 39373197

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-516489 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 492 amino acids
Theoretical weight: 54.47 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: K4FW35 (Residues: 1-471; Coverage: 100%)
Gene name: SHMT
Sequence domains: Serine hydroxymethyltransferase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 6 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P3121
Unit cell:
a: 174.175Å b: 174.175Å c: 183.658Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.227 0.266
Expression system: Escherichia coli

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