› 9cga

X-ray diffraction
2.01Å resolution

Human DJ-1, 3 sec mixing with methylglyoxal, pink beam time-resolved serial crystallography, CrystFEL processed

Released:
DOI: 10.2210/pdb9cga/pdb
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PDB entry 9cga coloured by chain, front view.
PDB entry 9cga coloured by chain, side view.
PDB entry 9cga coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Resolving DJ-1 Glyoxalase Catalysis Using Mix-and-Inject Serial Crystallography at a Synchrotron.
Zielinski KA, Dolamore C, Dalton KM, Smith N, Termini J, Henning R, Srajer V, Hekstra DR, Pollack L, Wilson MA
bioRxiv (2024)
PMID: 39071394

Function and Biology Details

Reaction catalysed: 
An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + lactate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parkinson disease protein 7 Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 20.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99497 (Residues: 1-189; Coverage: 100%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family

Ligands and Environments

1 bound ligand:
Ligand 4Y8 1 x 4Y8
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P3121
Unit cell:
a: 76.03Å b: 76.03Å c: 76.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.158 0.178
Expression system: Escherichia coli BL21(DE3)

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