› 9cgi

Electron Microscopy
2.92Å resolution

Cryo-EM structure of the Nipah Virus polymerase (L) protein in complex with the tetrameric phosphoprotein (P)

Released:
DOI: 10.2210/pdb9cgi/pdb
PDB entry 9cgi coloured by chain, front view.
PDB entry 9cgi coloured by chain, side view.
PDB entry 9cgi coloured by chain, top view.
Source organism: Henipavirus nipahense
Primary publication:
Structure of the Nipah virus polymerase phosphoprotein complex.
Yang G, Wang D, Liu B
Nat Commun 15 8673 (2024)
PMID: 39375338
Related structures: EMD-45580

Function and Biology Details

Reactions catalysed: 
(1a) S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] = S-adenosyl-L-homocysteine + G(5')pppAmACA-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
(1a) (5')pppAACA-[mRNA] + (protein L)-L-histidine = diphosphate + (protein L)-L-histidyl-(5')phosphonato-AACA-[mRNA] + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-275296 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 2244 amino acids
Theoretical weight: 257.57 KDa
Source organism: Henipavirus nipahense
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q997F0 (Residues: 1-2244; Coverage: 100%)
Gene name: L
Sequence domains:
Phosphoprotein Chains: B, C, D, E
Molecule details ›
Chains: B, C, D, E
Length: 709 amino acids
Theoretical weight: 78.39 KDa
Source organism: Henipavirus nipahense
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9IK91 (Residues: 1-709; Coverage: 100%)
Gene name: P/V/C
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.92Å
Relevant EMDB volumes: EMD-45580
Expression system: Spodoptera frugiperda

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