› 9cgu

Electron Microscopy
3Å resolution

Cas9 ternary complex, 14-nt sgRNA, State II (kinked)

Released:
DOI: 10.2210/pdb9cgu/pdb
PDB entry 9cgu coloured by chain, front view.
PDB entry 9cgu coloured by chain, side view.
PDB entry 9cgu coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of Cas9 DNA interrogation with a 5' truncated sgRNA.
Kiernan KA, Kwon J, Merrill BJ, Simonović M
Nucleic Acids Res (2024)
PMID: 39657754
Related structures: EMD-45586

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-281339 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1368 amino acids
Theoretical weight: 158.7 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
Target strand Chain: C
Molecule details ›
Chain: C
Length: 40 nucleotides
Theoretical weight: 12.18 KDa
Source organism: synthetic construct
Expression system: Not provided
Non-target strand Chain: D
sgRNA Chain: B

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-45586
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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