› 9cpo

Electron Microscopy
3.5Å resolution

Infectious bronchitis virus core polymerase complex

Released:
DOI: 10.2210/pdb9cpo/pdb
PDB entry 9cpo coloured by chain, front view.
PDB entry 9cpo coloured by chain, side view.
PDB entry 9cpo coloured by chain, top view.
Source organism: Infectious bronchitis virus
Primary publication:
A genus-specific nsp12 region impacts polymerase assembly in Alpha- and Gammacoronaviruses.
Hoferle PJ, Anderson TK, Kirchdoerfer RN
bioRxiv (2024)
PMID: 39091740
Related structures: EMD-45805

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269635 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
ORF1ab Chain: A
Molecule details ›
Chain: A
Length: 930 amino acids
Theoretical weight: 107.47 KDa
Source organism: Infectious bronchitis virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A8E8HBN7 (Residues: 3938-4867; Coverage: 14%)
Gene name: 1ab
Sequence domains:
ORF1ab Chains: B, D
Molecule details ›
Chains: B, D
Length: 195 amino acids
Theoretical weight: 21.57 KDa
Source organism: Infectious bronchitis virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A8E8HBN7 (Residues: 3470-3664; Coverage: 3%)
Gene name: 1ab
Sequence domains: Coronavirus replicase NSP8
ORF1ab Chain: C
Molecule details ›
Chain: C
Length: 72 amino acids
Theoretical weight: 8.01 KDa
Source organism: Infectious bronchitis virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A8E8HBN7 (Residues: 3383-3454; Coverage: 1%)
Gene name: 1ab
Sequence domains: Coronavirus replicase NSP7
RNA Primer Chain: P
Molecule details ›
Chain: P
Length: 30 nucleotides
Theoretical weight: 9.53 KDa
RNA template Chain: T
Molecule details ›
Chain: T
Length: 33 nucleotides
Theoretical weight: 10.57 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-45805
Expression system: Escherichia coli BL21

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