› 9cyo

X-ray diffraction
1.94Å resolution

Crystal structure of wild-type human PTP1B (PTPN1) at room temperature (298 K)

Released:
DOI: 10.2210/pdb9cyo/pdb
PDB entry 9cyo coloured by chain, front view.
PDB entry 9cyo coloured by chain, side view.
PDB entry 9cyo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of human PTP1B variants reveal allosteric sites to target for weight loss therapy.
Perdikari A, Woods VA, Ebrahim A, Lawler K, Bounds R, Singh NI, Mehlman TS, Riley BT, Sharma S, Morris JW, Keogh JM, Henning E, Smith M, Farooqi IS, Keedy DA
bioRxiv (2024)
PMID: 39149290

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 37.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-321; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase

Ligands and Environments

2 bound ligands:
Ligand BME 1 x BME
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-1
Spacegroup: P3121
Unit cell:
a: 89.541Å b: 89.541Å c: 106.212Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.17 0.202
Expression system: Escherichia coli

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