› 9e8g

Electron Microscopy
3.01Å resolution

Nub1/Fat10-processing human 26S proteasome bound to Txnl1 with Rpt5 at top of spiral staircase

Released:
DOI: 10.2210/pdb9e8g/pdb
PDB entry 9e8g coloured by chain, front view.
PDB entry 9e8g coloured by chain, side view.
PDB entry 9e8g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural landscape of AAA+ ATPase motor states in the substrate-degrading human 26S proteasome reveals conformation-specific binding of TXNL1.
Arkinson C, Gee CL, Zhang Z, Dong KC, Martin A
bioRxiv (2024)
PMID: 39574680
Related structures: EMD-47719

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
PDBe Complex ID:
PDB-CPX-281388 (preferred)
Entry contents:
35 distinct polypeptide molecules
Macromolecules (35 distinct):
26S proteasome regulatory subunit 7 Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 48.7 KDa
Source organism: Homo sapiens
26S proteasome regulatory subunit 4 Chain: B
Molecule details ›
Chain: B
Length: 440 amino acids
Theoretical weight: 49.26 KDa
Source organism: Homo sapiens
26S proteasome regulatory subunit 8 Chain: C
26S proteasome regulatory subunit 6B Chain: D
26S proteasome regulatory subunit 10B Chain: E
26S proteasome regulatory subunit 6A Chain: F
Proteasome subunit alpha type-6 Chain: G
Proteasome subunit alpha type-2 Chain: H
Proteasome subunit alpha type-4 Chain: I
Proteasome subunit alpha type-7 Chain: J
Proteasome subunit alpha type-5 Chain: K
Proteasome subunit alpha type-1 Chain: L
Proteasome subunit alpha type-3 Chain: M
Proteasome subunit beta type-6 Chain: N
Proteasome subunit beta type-7 Chain: P
Proteasome subunit beta type-3 Chain: Q
Proteasome subunit beta type-2 Chain: R
Proteasome subunit beta type-5 Chain: S
Proteasome subunit beta type-1 Chain: T
26S proteasome non-ATPase regulatory subunit 1 Chain: U
26S proteasome non-ATPase regulatory subunit 3 Chain: V
26S proteasome non-ATPase regulatory subunit 12 Chain: W
26S proteasome non-ATPase regulatory subunit 11 Chain: X
26S proteasome non-ATPase regulatory subunit 6 Chain: Y
26S proteasome non-ATPase regulatory subunit 7 Chain: Z
26S proteasome non-ATPase regulatory subunit 13 Chain: a
26S proteasome non-ATPase regulatory subunit 4 Chain: b
26S proteasome non-ATPase regulatory subunit 14 Chain: c
26S proteasome non-ATPase regulatory subunit 8 Chain: d
26S proteasome complex subunit SEM1 Chain: e
26S proteasome non-ATPase regulatory subunit 2 Chain: f
NEDD8 ultimate buster 1 Chain: g
Proteasome subunit beta type-4 Chain: h
Thioredoxin-like protein 1 Chain: u
substrate peptide Chain: v

Ligands and Environments

4 bound ligands:
Ligand ATP 3 x ATP
Ligand MG 5 x MG
Ligand ADP 3 x ADP
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.01Å
Relevant EMDB volumes: EMD-47719
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens

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